Fig. 4.

Involvement of Ffar2 in the reduction of NPP‐induced IL‐6 by inoculation of LCC onto S. epidermidis‐colonized mice (A) S. epidermidis‐colonized ICR mice were intranasally applied with LCC (+LCC) or PBS (‐LCC) 1 h before inoculation of NPP for 6 h. The level of IL‐6 in BALF induced by NPP was expressed as fold change over that induced by PBS. (B) S. epidermidis‐colonized ICR mice were given GLPG‐0974, a Ffar2 antagonist (+), or vehicle (ethanol) alone (‐) by gastric gavage every day for 3 days. After administration of GLPG‐0974, mice were intranasally applied with LCC six hours before inoculation with NPP. The level of IL‐6 in BALF of mice given with GLPG‐0974 was expressed as fold change over that in BALF of mice given without GLPG‐0974. The P‐value of < 0.001 (**); < 0.0001 (***) from three independent experiments with mean ± SD is shown.