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Sensitivity of multiplex PCR in detecting EHEC virulence factors using serial dilutions of E. coli O111:H−. DNA markers are indicated on the right (numbers are molecular weights, in base pairs). Lanes 1 through 13 contain 6,250, 2,500, 630, 372, 186, 93, 46, 37, 18, 9, 3, 1.5, and <1 genome equivalents, respectively.
