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Primer sequences and predicted lengths of PCR amplification products
| Primer | Direc-tion | Primer sequence (5′-3′) | Fragment size (bases) | Refer-ence |
|---|---|---|---|---|
| EHEC hly | Forward | ACGATGTGGTTTATTCTGGA | 165 | 12 |
| Reverse | CTTCACGTGACCATACATAT | |||
| stx1 | Forward | ACACTGGATGATCTCAGTGG | 614 | 13 |
| Reverse | CTGAATCCCCCTCCATTATG | |||
| stx2 | Forward | CCATGACAACGGACAGCAGTT | 779 | 13 |
| Reverse | CCTGTCAACTGAGCAGCACTTTG | |||
| eaeA | Forward | GTGGCGAATACTGGCGAGACT | 890 | 14 |
| Reverse | CCCCATTCTTTTTCACCGTCG |
