FIGURE 5.

RBD‐ and spike‐specific IgG and IgA were detected locally in BAL, with RBD‐specific IgG2b dominating the local subclass response (A–C) RBD‐ (A, C) and spike‐ (B, C) specific IgG titer in BAL for the groups vaccinated with CuMV_TT‐RBD vaccine or CuMV_TT control, measured by ELISA, OD₄₅₀ shown in A, B, LOG₁₀ OD₅₀ shown in C. (D–F) RBD‐ (D, F) and spike‐ (E, F) specific IgA titer in BAL for the groups vaccinated with CuMV_TT‐RBD vaccine or CuMV_TT control, measured by ELISA, OD₄₅₀ shown in D, E, LOG₁₀ OD₅₀ shown in F. (G) RBD‐specific IgG1, IgG2a, IgG2b, and IgG3 titer in BAL for the groups vaccinated with CuMV_TT‐RBD vaccine or CuMV_TT control, measured by ELISA, LOG₁₀ OD₅₀ shown. (H) RBD‐specific IgA titer in BAL from mice immunized with CuMV_TT‐RBD vaccine, LOG₁₀ OD₅₀ shown. Plates in duplicates: treated with PBSTween or 7 M urea. (I) Avidity index of RBD‐specific IgA titer. (J) Avidity indexes of RBD‐specific IgA Abs found in BAL and serum after CuMV_TT‐RBD immunization. Statistical analysis (mean ± SEM) using Student's t‐test. Control group n = 6, vaccine group n = 6. One representative of 2 similar experiments is shown