TABLE 2.
Peptides included in study
| Peptide name | Sequence | HLA allele | Immuno‐prevalence, % | Fluorochrome 1 a | Fluorochrome 2 a |
|---|---|---|---|---|---|
| S269‐277 | YLQPRTFLL | A*02:01 | ≥45 | SA‐APC (Invitrogen) | SA‐PE (Invitrogen) |
| S378‐387 | KCYGVSPTKL | A*03:01 | SA‐BV421 (Biolegend) | SA‐PE (Invitrogen) | |
| S89‐97 | GVYFASTEK | A*03:01 | SA‐APC (Invitrogen) | SA‐APC‐R700 (BD) | |
| S865‐874 | LTDEMIAQYT | A*01:01 | SA‐APC‐R700 (BD) | SA‐PE (Invitrogen) | |
| S1000‐1008 | RLQSLQTYV | A*02:01 | 13 | SA‐APC (Invitrogen) | SA‐PE‐CF594 (BD) |
| S367‐378 | VLYNSASFSTFK | A*03:01 | 11 | SA‐APC‐R700 (BD) | SA‐PE‐Cy5 (BD) |
Abbreviations: APC, allophycocyanin; BV421, brilliant violet 421; Cy5, cyanine 5; PE, R‐phycoerythrin; SA, streptavidin.
a
staining with dual fluorochrome combination.