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. 2022 May 10;20:138–146. doi: 10.1016/j.reth.2022.04.005

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Circ_ROBO2 knockdown alleviated ox-LDL-induced dysfunction in CMECs. (A) RT-qPCR was performed to evaluate the interference efficiency of sh-circ_ROBO2 in CMECs. (B–G) CMECs were divided into four groups: Control, ox-LDL, ox-LDL + sh-NC, and ox-LDL + sh-circ_ROBO2. (B) DNA synthesis was determined using an EdU assay. (C) Cell viability was assessed using CCK-8 assay. (D) The protein level of PCNA was detected by western blot assay. (E) The percentage of apoptotic CMECs was assessed by flow cytometry analysis. (F) The protein levels of Bcl-2 and Bax were analyzed by western blot assay. (G) Cell angiogenic ability was assessed by Matrigel tube formation assay. ∗P < 0.05.