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. 2022 May 10;20:138–146. doi: 10.1016/j.reth.2022.04.005

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — MiR-186-5p was a direct target of circ_ROBO2. (A) The binding sites of circ_ROBO2 and miR-186-5p were predicted by circinteractome. (B and C) Dual-luciferase reporter assay and RNA-pull down assay were performed to verify the target relationship between circ_ROBO2 and miR-186-5p. (D) The expression of miR-186-5p was detected by RT-qPCR in CMECs transfected with sh-NC or sh-circ_ROBO2. (E) The expression of miR-186-5p was measured by RT-qPCR in CMECs treated with or without ox-LDL. (F) The level of miR-186-5p was detected by RT-qPCR in serum samples of CAD patients and healthy controls. (G) The correlation between circ_ROBO2 and miR-186-5p in serum samples of CAD patients was analyzed. ∗P < 0.05.