Pramef12 enhances somatic cell reprogramming by acting on middle phase. Time course analysis of the expression of Nanog (A), Gtl2 (B), Slc2a1 (C), Pgk1 (D), Pdk1 (E), Cox7a1 (F), Idh2 (G), Esrrb (H), Zic3 (I), Snail (J), and Cdh1 (K) determined by RT-qPCR. Data are presented as the mean ± s.d. (n = 3). *P < 0.05, **P < 0.01 (Student's t-test). The numbers in parentheses indicate the number of days the cells were incubated with Dox.