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. 2022 May 17;15:170. doi: 10.1186/s13071-022-05286-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — IFA detection of GP900 protein in excysted sporozoites. a Dual-labeling of GP900 with mouse anti-GP900-N (red) and rabbit anti-GP900-C (green) in excysted sporozoites. b, c IFA detection of GP900 protein on the external surface of sporozoites that had been fixed in formaldehyde but were unpermeabilized (b) in comparison with permeabilized sporozoites (c), using anti-GP900-N antibody. To facilitate a good comparison, both samples were processed in parallel under the same experimental conditions. It should be noted that weak, but clear when over-exposed, signals were produced in unpermeabilized sporozoites. d, e IFA detection of GP900 protein on the trails of gliding sporozoites using anti-GP900-N (d) and anti-GP900-C (e) antibodies; anti-GP900-N antibody stained the surface of sporozoites and some gliding trails (d) while anti-GP900-C antibody only stained the apical region of the gliding sporozoites (e). In all panels, nuclei were stained with DAPI