Fig. 5.

Quantitative analysis of S. aureus mutants.A, pie chart showing the changes in pArg phosphorylation and volcano plot showing the quantitative changes in all phosphorylation sites between Stp1 mutant and Ctrl, identifying 411 changes (Tukey HSD p-value cutoff of 0.05 and a fold change cutoff of $\overline{x}\pm \sigma$ of the data). Quantified pArg sites are highlighted in purple and show a clear overrepresentation in the Stp1 mutant. Underrepresented p-sites are shown in pink and overrepresented sites in turquoise. Three pArg sites were underrepresented, and 72 pArg sites were overrepresented. B, pie chart showing the changes in pArg phosphorylation, and volcano plot comparing the phosphorylation sites between Stk1 mutant and Ctrl, identifying 117 changes (Tukey HSD p-value cutoff of 0.05 and a fold change cutoff of $\overline{x}\pm \sigma$ of the data). Seven pArg sites were underrepresented and five pArg sites overrepresented. All identified pArg sites are equally distributed between Stk1 mutant and Ctrl (purple dots).