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. 2022 Apr 12;21(5):100232. doi: 10.1016/j.mcpro.2022.100232

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 6 — Dephosphorylation of synthetic and endogenous phosphopeptides. Percentage of identified synthetic (A) pArg, (B) pSer/Thr, and (C) pTyr after incubation without phosphatase, recombinant Stp1, or shrimp alkaline phosphatase rSAP (n = 4). D, average number of identified peptides and phosphopeptides after incubation with or without Stp1. E, distribution of serine, threonine, tyrosine, histidine, and arginine phosphosites identified. The stack bars represent the average percentage of phosphosites (n = 3) and the standard deviation is shown as error bars. Numbers depict the number of identified sites.