Figure 5.

Conditional CRISPR-Cas9 system and human stem cell hepatocyte differentiation

(A) Diagram of conditional CRISPR-Cas9 system with various promoter systems. (B) H9-Cas9 clones that were replaced with EF1α and human albumin (hALB) promoters were differentiated into hepatocyte-like cells. Level of sgRNA targeting p53 gene was measured by RT-qPCR during hepatocyte differentiation. (C) CMV, EF1α, and hALB promoter activities in the conditional CRISPR-Cas9 system, as shown by the Nanoluciferase activities, were compared during hepatocyte differentiation of SW-Cas9 (iPSC) and H9-Cas9 (ESC) clones. Data are shown as mean values ±SEM of triplicate experiments; ns, not significant. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.