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. 2022 May 9;2022:4169150. doi: 10.1155/2022/4169150

Figure 11.

Figure 11

SHNG1 and SHNG3 inhibit apoptosis and promote proliferation of HCC cells. (a) Expression of SHNG1 in LO2, HepG2, Hep3B, and Huh7 cell lines was measured by qRT-PCR. (b) siRNA knockdown efficiency was confirmed by qRT-PCR in Hun7 cells. Huh7: Huh7 cells without transfection; Huh7-NC: Huh7 cells transfected with negative control (NC); Huh7-si-SHNG1: Huh7 cells transfected with si-SHNG1. (c) siRNA knockdown efficiency was confirmed by qRT-PCR in HepG2 cells. HepG2: HepG2 cells without transfection; HepG2-NC: HepG2 cells transfected with negative control; HepG2-si-SHNG3: Huh7 cells transfected with si-SHNG3. (d, e) Colony formation assay was used to assess the effect of siSHNG1 in Huh7 cells and the effect of siSHNG3 in HepG2 cells. (f, g) Flow cytometry analysis of Annexin V-FITC/PI staining was used to assess the number of apoptotic cells in each treatment in Huh7 and HepG2 cells. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.0001.