Figure 3.

Concentration–response curves of the agonists adenosine, NECA and BAY 60-6583 at HEK293 cells transiently transfected with TRUPATH biosensors for the indicated G protein subunits. A-D. ΔBRET values measured in HEK293 cells with native A_2BAR expression levels and overexpression of the indicated biosensors A. Gα_q, B. Gα₁₁, C. Gα₁₅, or D. Gα_s-s. E-H. ΔBRET values measured in HEK293 cells with overexpression of the A_2BAR and the indicated biosensors Gα_q (e), Gα₁₁ (f), Gα₁₅ (g), or Gα_s-s (h) biosensors. Data points are presented as means ± SEM of three or more independent experiments; pEC₅₀ values and efficacy values are listed in Tables S2 and S3 of Supporting Information.