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. 2022 May 16;14:63. doi: 10.1186/s13148-022-01282-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Gene body methylation drives expression of PRKAR1B/HEATR2. A Methylation levels at individual CG sites covered by the Illumina 450 K array in WT (HCT116, blue) and knockout (DKO, orange) cells within PRKAR1B gene with highlighted CGI within promoter (green bar). B Data as in A for the HEATR2 gene . C DNA methylation differences at PRKAR1B/HEATR2 promoter assessed by pyroassay in WT (HCT116) and DKO cells (blue) and D SHSY5Y WT and Aza (orange) -treated cells. E DNA methylation levels at CG sites located within GB of PRKAR1B and F HEATR2 covered by pyroassay in WT (HCT116) and knockout (DKO cells). Values are shown as mean +/− SD for each site: *p < 0.05; **p < 0.01; ***p < 0.001. RT-qPCR of PRKAR1B (G) and HEATR2 (H) in HCT116 and SHSY5Y (I, J) cell lines. Location of pyroassays and RT-qPCR products used for this figure are indicated in Fig. 3A. Values are shown as mean +/− SD: *p < 0.05; **p < 0.01; ***p < 0.001