Table 3.

Primer sets used for pyrosequencing and transcriptional analysis used in this study, including RT-qPCR

Application	Name	Oligo sequence (5′–3′)
Pyrosequencing	pyro_MIR4520A_FW	GTTTAAATTTTTTTTTGATTTGGATAGAAA
	pyro_MIR4520A_RV	AAAACATACCCTCAATTCCAAAAAAAT C
	pyro_MIR4520A_Seq	TTTTTTTTGATTTGGATAGAAAATA
	pyro_PRKAR1b/HEATR2_Promoter_FW	TTTAGGGGTAGGTTTAGGTTTATAGT
	pyro_PRKAR1b/HEATR2_Promoter_RV	CCAACCTACCTACTAAACCTTATC
	pyro_PRKAR1b/HEATR2_Promoter_Seq	GGTAGGTTTAGGTTTATAGTT
	pyro_PRKAR1b_GB_FW	GGTTTTATAGGGATGAGGTTTTATGTATAG
	pyro_PRKAR1b_GB_RV	ACCACCCACAACATCAAACAAA
	pyro_PRKAR1b_GB_Seq	AGGTTTTATGTATAGGGTAT
	pyro_HEATR2_GB_FW	GGTAGGGAGGTATTTGGTTTAAGA
	pyro_HEATR2_GB_RV	TAAAACCCCCCCACCTACCCTTACC
	pyro_HEATR2_GB_Seq	GGGAGGTATTTGGTTTAAGATT
	pyro_DUSP22_FW	GTGAGGTTTGAGGTTAGAGATTTAG
	pyro_DUSP22_RV	ATCTCCAAATCCCCCTTTAACC
	pyro_DUSP22_Seq	TTTAGGGTAGGGAGG
	pyro_CES1_FW	GGTTGGTCGGGTTTAGTTGTTTTAAG
	pyro_CES1_RV	AAACAACACAATCCCTCTAAACTAC
	pyro_CES1_Seq	TTAAGTTTAAGTTTTAATATGGAA
RT-qPCR	qPCR_PRKAR1b_GB_FW	ACAGGGGAGTGAGGTGAGTG
	qPCR_PRKAR1b_GB_RV	TCTTTGAGGACCTGCTGGAT
	qPCR_HEATR2_FW	AGCCCTGGCGTCGTGATTAGT
	qPCR_HEATR2_RV	CCCGTTGAGCACACAGAGGCCTA
	qPCR_DUSP22_FW	CGCTGACTTGTTGACACTGC
	qPCR_DUSP22_RV	GCTCAATTGTTCCGCGTCTC
	qPCR_CES1_FW	TTGCTGCCCATGAAAACGTG
	qPCR_CES1_RV	GCATTGGAATCAACCAGCC
	HPRT_FW	AGCCCTGGCGTCGTGATTAGT
	HPRT_RV	CCCGTTGAGCACACAGAGGCCTA

Pyroassay primers are given as bisulphite-converted