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. 2022 May 17;18(5):e10947. doi: 10.15252/msb.202210947

Figure 4. Quantitative analysis of sub‐cellular proteomes and biological pathways.

Figure 4

  1. Total protein copy number of the oxidative phosphorylation complex I–V in bulk liver tissue (n = 6 independent biological replicates) and primary cell types (isolated primary cells from n = 3 individuals for each cell type). Values are presented as mean ± s.d.
  2. Protein copy number for members of the oxidative phosphorylation complex I–V in hepatocytes and Kupffer cells with Pearson correlation coefficient indicated.
  3. Protein copy number estimation of the KEGG pathways. The left panel shows the estimated total number of proteins per cell of top 10 most abundant pathways in liver biopsy in terms of total molecules of proteins associated. The right panel shows the protein copy numbers of top 10 most abundant proteins in liver biopsy that are associated with metabolic pathways and their distribution in liver biopsy (n = 6), hHEP (n = 3), hLSEC (n = 3), hHSC (n = 3), and hKC (n = 3). The black line in the middle of the box is the median, the top and the bottom of the box represent the upper and lower quartile values of the data and the whiskers represent the upper and lower limits for considering outliers (Q3+1.5*IQR, Q1−1.5*IQR) where IQR is the interquartile range (Q3–Q1).
  4. Top 10 uniquely quantified proteins per cell type with label‐free quantification (LFQ) intensities [Log10] and copy numbers [Log10].