Table 1.
Summary of modifications in membrane composition in Caco-2 cells exposed to NM-103 and NM-104 at a concentration of 3.13 µg/cm2 for 1 week, 2 weeks and for 1 week with a 1-week recovery period
| Caco-2 | NM103 | NM104 | |||
|---|---|---|---|---|---|
| Ion m/z | Putative identity | Effect | Recoverable | Effect | Recoverable |
| 177 | Asparagine | ↑ | ✓ | ND | ND |
| 179 | Alanine | ↑ | ✓ | ND | ND |
| 493 | Lyso-sphingomyelin (d20:1) | ↑ (2 weeks) | – | ND | ND |
| 495 | Lyso-sphingomyelin (d20:0) | ↑ (2 weeks) | – | ND | ND |
| 531 | Lyso-phosphatidylglycerol (C20:5) | ND | ND | ↓ | ✓ |
| 545 | Lyso-phosphatidylinositol (C14:0) | ND | ND | ↓ | ✓ |
| 557 | Lyso-phosphatidylglycerol (C22:6) | ND | ND | ↓ | ✓ |
| 571 | Lyso-phosphatidylinositol (C16:1) | ND | ND | ↓ | ✓ |
| 625 | Lyso-phosphatidylinositol (C20:2) | ↓ | ✗ | ↓ | ✗ |
| 629 | Lyso-phosphatidylinositol (C20:0) | ↓ | ✗ | ↓ | ✗ |
| 687 | Phosphatidylglycerol (C30:3) | ↓ | ✗ | ↓ | ✗ |
| 715 | phosphatidylglycerol (C32:4) | ↓ | ✗ | ↓ | ✗ |
Presented ions represent ion yields for characteristic biomarker ions which were used to separate the treatment groups