TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristicsa | Source or referenceb |
|---|---|---|
| Strains | ||
| E. coli ER2275 | hsdR mcr recA1 endA1 | NEB |
| C. acetobutylicum ATCC 824 | Wild type | ATCC |
| Plasmids | ||
| pN5-1 | ptb buk | 59 |
| pIMP1 | ColE1 ORI, Apr, pIM13 ORI, MLSr | 33 |
| pSOS94 | ColE1 ORI, Apr, pIM13 ORI, MLSr, ptb promoter region | 49 |
| pRD1 | ColE1 ORI, Apr, pIM13 ORI, MLSr, ptb-asRNA | This study |
| pRD4 | ColE1 ORI, Apr, pIM13 ORI, MLSr, buk-asRNA | This study |
a
hsdR, host-specific restriction deficient; mcr, methylcytosine-specific restriction abolished; recA1, homologous recombination abolished; endA1, endonuclease abolished; ColE1 ORI, ColE1 origin of replication; Apr, ampicillin resistance; pIM13 ORI, gram-positive origin of replication; MLSr, macrolide-lincosamide-streptogramin resistance; ptb, PTB gene; buk, BK gene.
b
NEB, New England Biolabs, Beverly, Mass.; ATCC, American Type Culture Collection, Manassas, Va.