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Purification of gentisate 1,2-dioxygenase from P. putida P35X
| Purification step | Volume (ml) | Total activity (U) | Total protein (mg) | Sp act (U/mg) | Yield (%) | Purifi-cation (fold) |
|---|---|---|---|---|---|---|
| Crude extract | 50 | 2,473 | 3,435 | 0.72 | 100.00 | 1.00 |
| Heat treatment (60°C) | 41 | 1,562 | 1,838 | 0.85 | 63.20 | 1.18 |
| 40–55% (NH4)2SO4 fractionation | 8.3 | 1,175 | 1,049 | 1.12 | 47.51 | 1.56 |
| DEAE-Sepharose CL-6B | 40 | 847 | 83 | 10.20 | 34.25 | 14.17 |
| Mini Prep cell | 8 | 220 | 5.1 | 43.14 | 8.90 | 59.92 |
