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Effects of some metal ions on P25X and P35X gentisate 1,2-dioxygenases
| Metal ion (5 mM) | Relative activity (%)a
|
|
|---|---|---|
| P25X | P35X | |
| None | 100.00 | 100.00 |
| NaCl | 98.33 | 106.02 |
| KCl | 95.70 | 107.76 |
| MgCl2 | 94.62 | 66.00 |
| CaCl2 | 97.63 | 101.00 |
| MnSO4 | 73.96 | 22.36 |
| ZnSO4 | 2.58 | 4.69 |
| CuSO4 | 0.00 | 0.00 |
| EDTA | 60.24 | 4.95 |
Enzymes were preincubated with 5 mM different metal ions for 15 min before the activities were assayed. The activity of the enzyme in the absence of any metal ion was considered as 100% activity.
