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. Author manuscript; available in PMC: 2023 Jun 15.
Published in final edited form as: Life Sci. 2022 Apr 2;299:120528. doi: 10.1016/j.lfs.2022.120528

Figure 2. There is significant inflammation in the bladder of diabetic mice which is not present when NLRP3 is deleted.

Figure 2.

The Evans blue dye extravasation assays was used to assess inflammation in the bladder of the Akita mouse in the presence of NLRP3 or with NLRP3 genetically deleted. A. Diabetes significantly increased the amount of Evans blue dye in the bladder compared to control at 30 weeks when NLRP3 was intact (NLRP+/+). n = 10 (nondiabetic) and 3 (diabetic). B. Diabetes did not affect the movement of Evans blue into the bladder in the absence of NLRP3. n = 5 (nondiabetic) and 12 (diabetic). For all graphs bars represent the mean ± SEM. ***P < 0.0001 by paired Student two-tailed t test. non-diab = nondiabetic. diab = diabetic. ANOVA followed by Student-Newman-Keuls post hoc test was also used to compare all groups. The only additional significant differences found were between diabetic/NLRP3+/+ and nondiabetic/NLRP3−/− as well as diabetic/NLRP3−/−. These comparisons can be found in the supplemental material.