Figure 3. There is significant hypertrophy of the smooth muscle layer in the bladder of diabetic mice which is not present when NLRP3 is deleted.

Whole bladders from each group with harvested, fixed, embedded, sectioned and sections (5 μm) stained for Hematoxylin and Eosin and visualized using standard methods, as described in the methods section. SM = bladder smooth muscle; U = urothelia; L = lumen. The lumen of the bladder is necessarily collapsed (i.e. not inflated) and is only represented by small while areas. Error bars represent 50 μm.