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. 2022 Apr 7;204(5):e00105-22. doi: 10.1128/jb.00105-22

FIG 2.

FIG 2

Cascade is nucleoid-enriched in a crRNA-dependent manner while Cas3 is cytoplasmic. (a) A model for DNA damage-induced nucleoid compaction. (b) Fluorescence microscopy of nucleoid compacted wild-type or ΔcrRNA cells expressing Csy1-sfCherry or Csy4-sfCherry and nucleoid compacted wild-type cells expressing Cas3-sfCherry. Fluorescence intensity maps plotted against the long cell axis are shown. *, indicates which cell is plotted in the map. (c) Fluorescence microscopy of nucleoid-compacted ΔCas-crRNA cells expressing Csy1-sfCherry or Csy4-sfCherry from a plasmid. (d) Fluorescence microscopy of nucleoid-compacted ΔcrRNA cells expressing Csy1-sfCherry and synthetic crRNAs. Scale bars, 1 μm. For b and c, more than 125 nucleoid-compacted cells from each genotype were analyzed from two independent experiments.