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AcrIF2 but not AcrIF1 or AcrIF3 blocks nucleoid localization of type I-F Cascade. Fluorescence microscopy of nucleoid-compacted wild-type cells producing Csy1-sfCherry and expressing anti-CRISPR proteins AcrIF1, AcrIF2, or AcrIF3 from a plasmid (a) or a prophage (b). For a, more than 125 nucleoid-compacted cells from each genotype were analyzed from two independent experiments.
