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Nucleoid localization is conserved for SpyCas9. Fluorescence microscopy of nucleoid-compacted P. aeruginosa PAO1 cells producing Cas9-sgRNA, ApoCas9, or Cas9-sgRNA coexpressed with anti-CRISPR protein AcrIIA4. Fluorescence intensity maps plotted against the long cell axis are shown. Scale bar, 1 μm.
