FIG 2.

In vitro growth phenotypes of Δglby. (A) Growth of parent strain Mab ATCC 19977 (WT), Δglby and Complement (COMP) strains in Middlebrook 7H9 broth at 96 h, 37°C, constant shaking at 200 RPM. Culture media only as negative controls (NC) was also included. (B) Time course of optical densities of cultures of WT, Δglby, and COMP measured as absorbance at 600 nm. Each time point represents the average from four distinct replicates and no sample tube was used more than once. Error bars are standard deviation of each sample. (C) Time course of CFU of the three strains. Error bars are standard deviation of each sample. (D) Generation time (time required for a strain to double in CFU) of each strain determined from exponential phase(s) of growth. Two generation times reported for Δglby represent the biphasic exponential growth stages of this strain.