FIG 5.

Activity of Glby against Nitrocefin. Time course of nitrocefin (100 µM) hydrolysis by BlaC, Glby, RAD6, and ubiquitin (1.7 µM, each protein) was measured at 490-nm wavelength over a period of 360 min. Each data point shown is the mean of triplicates. To account for the low level of hydrolysis of nitrocefin in the reaction buffer, the absorbance in the control reaction (nitrocefin only) at each time point was used to correct the absorbance readings in reactions containing protein. As RAD6 and ubiquitin failed to hydrolyze nitrocefin, their plots appear superimposed.