FIG 1.

Loss of Set1-mediated mono-, di-, and trimethylation at histone H3K4 in S. cerevisiae and C. glabrata results in increased azole susceptibility and delayed growth in vitro. (A) Fivefold serial dilution spot assays of the indicated S. cerevisiae strains were grown on SC media with and without 8 μg/mL fluconazole and incubated at 30°C for 72 h. (B and D) Whole-cell extracts isolated from the indicated strains were immunoblotted using histone H3K4 methyl-specific mono-, di-, and trimethylation antibodies of whole-cell extracts isolated from the indicated strains. Histone H3 was used as a loading control. (C) Fivefold serial dilution spot assays of the indicated C. glabrata strains were grown on SC media with or without 32 μg/mL fluconazole and incubated at 30°C for 48 h. (E) Liquid growth curve assay of the indicated C. glabrata strains grown over 50 h with or without 32 μg/mL fluconazole.