FIG 2.

Deletion of Set1 complex members in C. glabrata results in increased azole susceptibility and loss of histone H3K4 methylation. (A) MIC assay of the indicated strains performed in RPMI 1640 media at 35°C, and results recorded after 48 h of incubation. (B) Fivefold serial dilution spot assays of the indicated C. glabrata strains were grown on SC plates with or without 32 μg/mL fluconazole. (C) Whole-cell extracts isolated from the indicated strains were immunoblotted using H3K4 methyl-specific mono-, di-, and trimethylation antibodies. Histone H3 was used as a loading control.