FIG 3.

The catalytic activity of the SET domain is necessary for Set1-mediated histone H3K4 methylation and alters azole susceptibility in C. glabrata. (A) Sequence alignment comparing the SET domain amino acid residues of S. cerevisiae and C. glabrata Set1. Asterisks indicate residues required for catalytic activity. (B) Fivefold serial dilution spot assays of the indicated C. glabrata strains were grown on SC plates with or without 32 μg/mL fluconazole. (C) Whole-cell extracts isolated from the indicated strains were immunoblotted using methyl-specific mono-, di-, and trimethylation antibodies. Histone H3 was used as a loading control.