FIG 4.

Drug efflux pump expression and drug efflux is altered in a Scset1Δ but not Cgset1Δ. (A and B; D and E) Expression of the indicated genes was determined in ScWT, Scset1Δ, CgWT, and Cgset1Δ strain cells treated with or without 64 μg/mL fluconazole for 3 h by qRT-PCR analysis. Gene expression analysis was set relative to the untreated wild type, and expression was normalized to RDN18 mRNA levels. Data were analyzed from ≥3 biological replicates with three technical replicates each. Error bars represent the standard deviations (SD). (C and F) Red fluorescence units (RFU) were measured as output in a Nile red assay to determine the amount of drug efflux in the indicated strains with and without fluconazole. Scpdr5Δ and Cgpdr1Δ strains was used as controls. Data were analyzed from ≥3 biological replicates with three technical replicates each. Statistics were determined using the GraphPad Prism Student t test, version 9.2.0. ns, P > 0.05; *, P < 0.05; ****, P < 0.0001. Error bars represent SD.