FIG 6.
Set1-mediated histone H3K4 methylation is enriched on ERG gene chromatin and is required for azole induction of ERG genes. (A and B) ChIP analysis of histone H3K4 trimethylation levels at the promoter, 5′, and 3′ regions of ERG11 and ERG3 in a wild-type C. glabrata strain with or without 64 μg/mL fluconazole treatment. ChIP analysis was set relative to a set1Δ strain and normalized to histone H3 and DNA input levels. Data were analyzed from five biological replicates with three technical replicates each. *, P < 0.05. (C and D) Expression of indicated genes was determined in the indicated mutants treated with and without 64 μg/mL fluconazole for 3 h by qRT-PCR analysis. Gene expression analysis was set relative to the untreated wild-type containing an empty vector and expression was normalized to RDN18 mRNA levels. Data were analyzed from ≥3 biological replicates with three technical replicates each. Statistics were determined using the GraphPad Prism Student t test, version 9.2.0. ns, P > 0.05; *, P < 0.05; **, P < 0.01. Error bars represent the SD.