Skip to main content
. 2022 Apr 26;66(5):e02250-21. doi: 10.1128/aac.02250-21

FIG 7.

FIG 7

Loss of SET1 in C. glabrata results in reduced endogenous ergosterol levels upon azole treatment. (A and B) Fivefold serial dilution spot assays of the indicated S. cerevisiae and C. glabrata strains were grown on SC plates with or without 8 or 32 μg/mL fluconazole, respectively, and 10 or 20 μg/mL ergosterol, respectively. (C to E) Total ergosterol was extracted from C. glabrata WT and set1Δ strains treated with or without 64 μg/mL fluconazole and analyzed by HPLC. The figure is depicted as a ratio of ergosterol to cholesterol and relative to untreated or treated WT. Data were generated from six biological replicates. Statistics were determined using the GraphPad Prism Student t test, version 9.2.0. ****, P < 0.0001; **, P < 0.01. Error bars represent the SD.