TABLE 2.

Mass spectrometry results of all proteins pulled down by DHA-biotin from both PfATP6-expressing whole yeast and PfATP6-enriched microsomes^a

Unassigned proteins	Mitochondrial proteins	Proteins also identified in P. falciparum	Proteins also identified in yeast protein-enriched vesicles
Adh1p alcohol dehydrogenase Ahp1p Arc1p tRNA delivery Bgl2p endo-β-1,3-glucanase Bmh1p Bub2p Dur1,2p urea carboxylase/allophanate hydrolase Erg20p synthesizing Rer2p substrate farnesyl diphosphate Gag Nam9p Pdc1p peroxisomal protein of unknown function Rnr2p ribonucleotide-diphosphate reductase subunit RNR2 Rpl10p ribosomal, chaperone Rpl11ap Rpl16bp Rpl9ap Rps14ap Ubi4p ubiquitin binding Ugp1p UTP glucose-1-phosphate uridylyltransferase Uncharacterized protein Wtm1p transcriptional modulator	6-Phosphogluconate dehydrogenase Acc1p acetyl-coenzyme A carboxylase Acetyl-coenzyme A synthetase ATP synthase subunit α Cyc1p po-cytochrome c Cytochrome c oxidase subunit 2 (fragment) Glutamate decarboxylase Glyceraldehyde-3-phosphate dehydrogenase Malate dehydrogenase Pck1p phosphoenolpyruvate carboxykinase PCK1 Pet9p ADP/ATP transporter Phosphoglycerate kinase Por1p mitochondrial outer membrane protein of unknown function Pyruvate carboxylase Pyruvate kinase Qcr2p ubiquinol-cytochrome c reductase subunit 2 Succinate dehydrogenase Superoxide dismutase [Cu-Zn] Triosephosphate isomerase	40S ribosomal protein S21 40S ribosomal protein S81 PfATP6 Act1p actin ATP-dependent 6-phosphofructokinase Elongation factor 1α Eno2p Eukaryotic translation initiation factor 5A Hsp104p Hsp12p Hsp26p Hsp30p Ribosomal protein L19 Rps18ap ribosomal subunit protein Ssa1p ATPase HSP70 family	PfATP6 Arc1p tRNA delivery Elongation factor 1α Hsp30p Plasma membrane ATPase DNA-directed RNA polymerase I subunit RPA43 Rps11bp Sec28p coatomer subunit ε Yro2p

^aResults are separated into unassigned proteins, mitochondrial proteins, proteins also pulled down from P. falciparum and human cancer cells in other studies, and proteins identified in yeast microsomes. The presence of PfATP6 is highlighted in bold.