FIG 2.
Detailed view of the integron-like element carrying GES and OXA β-lactamase genes in isolate CLB 24388. Open reading frames (ORFs) are represented by arrows. This segment (GenBank accession no. JAFFGY010000016.1, bp 400,688 to 410,007) contains the genes encoding a DDE-type integrase and a class 1 integron integrase (intI1), indicated by green arrows. Antimicrobial resistance genes are shown with red arrows. They include sul1, qacEΔ1, aac(3)-I family gene, aac(6′)-Ib4, blaOXA-5, an additional aac(6′)-Ib4, and blaGES-2. The deposited JAFFGY010000016.1 contig contains an assembly stop gap between blaGES-2 and blaOXA-5. PCR amplification, cloning, and sequencing of the stop gap sequence (see the supplemental material) identified an additional full-length aac(6′)-Ib4 gene with an alternative valine start codon.