TABLE 3.

Genes coding for β-lactam resistance determinants as detected by WGS of P. aeruginosa CLB 24388 and EM 2972704

Isolate	MLST	β-lactamases			AmpC regulators			OprD
		PDC	GES	OXA	AmpD	AmpR	DacB (PBP4)
PAO1	549	PDC-1	NA	OXA-50	WT	WT	WT	WT
CLB 24388	17	PDC-8	GES-2	OXA-5, OXA-50	A136V, G148A	WT	WT	Type T1-VIa-like, with 66 divergent aa after G388
EM 2972704	309	PDC-19a	GES-19, GES-20	OXA-2 and OXA-50 A8T, T16A, K112E	Q44H, G148A	G283E, M288R	WT	Type T1-VII-like,a interrupted by ISPa1328 element containing a transposase gene after aa D43

^aOprD was interrupted after aa D43 by an ISPa1328 element containing a transposase (tnp) gene, followed by the continuation of OprD that is type T1-VII, differing by the following substitutions: starting the continuation of OprD there is a repeat insertion of 42L and 43D, P186G, 372(VDSSSS-YAGL-)383, and E437D (17). P. aeruginosa PAO1 genome sequence NC_002516.2 was used as the reference genome. Reference comparator amino acid sequences from PAO1 (WT, wild type) are the following: PDC-1, NP_252799.1; OXA-50, NP_254201.1; AmpD, NP_253212.1; AmpR, NP_252798.1; DacB, NP_251737; OprD, NP_249649.1. Reference sequences for other β-lactamases present are the following: PDC-8, WP_003118203.1; PDC-19, AEM44530.1; OXA-5, WP_032488483.1; OXA-2, WP_001007673.1; GES-2, WP_045912044.1; GES-19, WP_015059045.1; GES-20, WP_015059046.1.