TABLE 1.
Curated surface-regulated genes
| PA no. | Gene name | Gene product | Fold change compared to 5 mina |
|||
|---|---|---|---|---|---|---|
| 15 min | 30 min | 45 min | 60 min | |||
| PA0034 | Two-component response regulator | 2.08 | ||||
| PA0155 | pcaR | Transcriptional regulator | 2.56 | |||
| PA0159 | Transcriptional regulator | 2.474 | 2 | 2.368 | 2.211 | |
| PA0177 | Purine-binding chemotaxis protein | 3.042 | 5.75 | |||
| PA0178 | Two-component sensor | 3 | ||||
| PA0179 | Two-component response regulator | 3.258 | ||||
| PA0180 | cttP | Chemotactic transducer for trichloroethylene | 2.526 | |||
| PA0289 | gpuR | Transcriptional activator | 2 | |||
| PA0294 | aguR | Transcriptional regulator | 2.065 | |||
| PA0306a | Transcriptional regulator | 2.432 | 4.198 | |||
| PA0355 | pfpI | Protease | 2.684 | 6.632 | ||
| PA0499 | Pilus assembly chaperone | 2.852 | 4.296 | 3 | 3.259 | |
| PA0520 | nirQ | Regulatory protein | 2.711 | 3.092 | 3.513 | |
| PA0797 | Transcriptional regulator | 2 | ||||
| PA0962 | dps | DNA-binding stress protein, starved cells | 2.586 | 2.387 | ||
| PA1001 | phnA | Anthranilate synthase component I | 0.257 | 0.183 | ||
| PA1179 | phoP | Two-component response regulator | 0.307 | |||
| PA1180 | phoQ | Two-component sensor kinase | 0.331 | |||
| PA1347 | Transcriptional regulator | 2.185 | 2.074 | |||
| PA1603 | Transcriptional regulator | 2.514 | 2.73 | |||
| PA1898 | qscR | Quorum-sensing control repressor | 2.25 | 2.406 | 2.188 | |
| PA1930 | Chemotaxis transducer | 4.118 | ||||
| PA2016 | liuR | Regulator of liu genes | 2.839 | 4.361 | 3.078 | |
| PA2028 | Transcriptional regulator | 2.059 | ||||
| PA2191 | exoY | Adenylate cyclase | 2.348 | |||
| PA2259 | ptxS | Transcriptional regulator | 3 | |||
| PA2276 | Transcriptional regulator | 3.333 | ||||
| PA2788 | Chemotaxis transducer | 2.549 | ||||
| PA3120 | leuD | Isopropylmalate dehydratase | 2.180 | |||
| PA3477 | rhlR | Transcriptional regulator | 2.411 | 2.224 | ||
| PA3757 | nagR | Transcriptional regulator | 3.294 | |||
| PA3895 | Transcriptional regulator | 2.065 | ||||
| PA3916 | moaE | Molybdopterin-converting factor | 0.217 | |||
| PA4296 | pprB | Two-component response regulator | 2.538 | 2.442 | 4.038 | |
| PA4309 | pctA | Chemotactic transducer | 2.273 | 2.273 | ||
| PA4499 | psdR | Transcriptional regulator | 2.69 | |||
| PA4659 | Transcriptional regulator | 2.565 | 3.348 | 3.739 | ||
| PA4876 | osmE | osmE family transcriptional regulator | 3.742 | |||
| PA4878 | brlR | Transcriptional regulator | 2.707 | 2.576 | 2.22 | 2.271 |
| PA4915 | Chemotaxis transducer | 2.5 | 3.75 | |||
| PA5356 | glcC | DNA-binding transcriptional regulator | 2.37 | |||
| PA5365 | phoU | Phosphate uptake regulatory protein | 2.182 | 2.864 | 2.545 | |
a
Fold changes of genes at the indicated time points compared to the 5-min sample, where a fold change of 1 indicates no difference, a fold change of >1 indicates genes with elevated RNA levels compared to those at 5 min, and a fold change of <1 indicates genes with reduced RNA levels compared to those at 5 min (underlined). Empty cells indicate that there is no significant difference in gene expression compared to that at 5 min. Boldface type indicates the genes for which follow-up biofilm and complementation assays were performed (Fig. 5).