TABLE 2.

Genes that were differentially expressed across all three surfaces

PA no.	Gene name	Gene product	Fold expression difference (30 min [surface] vs 5 min postattachmenta)
			Plasticb	Silicone	Glass
PA0567		Hypothetical protein	0.684	0.200	0.411
PA0623		Bacteriophage protein	0.676	0.214	2.048
PA0839		Transcriptional regulator	0.590	0.000	0.252
PA0996	pqsA	Coenzyme A ligase	0.262	0.000	0.274
PA1985	pqqA	Coenzyme PQQ synthesis protein PqqA	0.659	0.519	0.317
PA2663	ppyR	psl and pyoverdine operon regulator, PpyR	0.669	0.000	0.218
PA2664	fhp	Nitric oxide dioxygenase	0.164	0.000	0.059
PA2759		Hypothetical protein	4.592	0.398	3.766
PA3205		Hypothetical protein	2.865	2.683	2.746
PA3871		PpiC-type peptidyl-prolyl cis-trans isomerase	23.313	2.875	15.000
PA3872	narI	Respiratory nitrate reductase subunit gamma	23.238	2.524	13.333
PA3877	narK1	Nitrite extrusion protein 1	0.146	0.013	0.123
PA3914	moeA1	Molybdenum cofactor biosynthetic protein A1	0.269	0.019	0.298
PA3915	moaB1	Molybdopterin biosynthetic protein B1	0.092	0.014	0.070
PA3916	moaE	Molybdopterin-converting factor large subunit	0.234	0.127	0.243
PA3917	moaD	Molybdopterin-converting factor small subunit	0.282	0.167	0.211
PA3918	moaC	Molybdenum cofactor biosynthesis protein MoaC	0.230	0.108	0.225
PA4270.1			2.201	2.007	2.716
PA4637a		Hypothetical protein	0.331	0.209	0.324
PA5369	pstS	Phosphate ABC transporter substrate-binding protein	3.353	2.294	3.529

^aFold changes of genes at 30 min postattachment on the indicated surfaces compared to those at 5 min postattachment on an Ibidi μ-Slide, where a fold change of 1 indicates no difference, a fold change of >1 indicates genes with elevated RNA levels compared to those at 5 min, and a fold change of <1indicates genes with reduced RNA levels compared to those at 5 min.

^bPolycarbonate plastic.