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Resistance formation by serial passaging. Six Gardnerella strains were passaged for up to 25 days in the presence of metronidazole (MDZ) or the hydroxy metabolite of MDZ (MDZ-OH) at sub-MICs, as indicated in the figure key. One representative Gardnerella strain was passaged with PM-477 for 25 days, as indicated in the figure key. The MIC (μg/mL) was determined every day prior to the next round of passaging. T, after strain designation indicates a type strain.
