FIG 6.
Purified cell-free Psl is sufficient to kill S. aureus. Survival of S. aureus grown in media supplemented with (A) increasing concentrations of purified cell-free Psl from PAO1 or the pagP::Tn mutant or cell-free products from the ΔPpsl mutant or (B) 60 μg/mL of d-mannose (Man), 20 μg/mL of d-glucose (Glu), 20 μg/mL of l-rhamnose (Rha), or a combination of the three monosaccharides (Combined). S. aureus grown without cell-free Psl or monosaccharides (untreated) was used as a control. OD600 was measured after 16 h. S. aureus survival is presented as OD600 normalized to that of the control. (A) The x axis is a log scale. Data are means and SD; individual points indicate the mean of biological replicates (N = 3; n = 3). Significance was determined using Student’s t test compared to the ΔPpsl mutant. *, P < 0.05.