TABLE 1.
Strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli | ||
| NEB5α | New England Biolabs | |
| S17 | ||
| P. aeruginosa | ||
| PAO1 | WT P. aeruginosa | 55 |
| ΔPpsl mutant | psl production deficient; psl operon promoter deletion mutant | 55 |
| ΔpslD/V mutant | psl production deficient; ΔpslD with an empty vector pUCP18 | 24 |
| ΔpslD/pslD+ mutant | pUCP18::pslD was used to complement ΔpslD | 24 |
| PAO1/V | WT P. aeruginosa with an empty vector pUCP18 | 24 |
| pagP::Tn mutant | pagP transposon mutant (UWGC:PW3442, PA1343::ISlacZ/hah) | 27 |
| htrB1::Tn mutant | htrB1 transposon mutant (UWGC:PW1009, PA0011::ISlacZ/hah) | 27 |
| htrB2::Tn mutant | htrB2 transposon mutant (UWGC:PW6435, PA3242::ISlacZ/hah) | 27 |
| lpxO1::Tn mutant | lpxO1 transposon mutant (UWGC:PW8596, PA4512::ISphoA/hah) | 27 |
| lpxO2::Tn mutant | lpxO2 transposon mutant (UWGC:PW2702, PA0936::ISphoA/hah) | 27 |
| pagL::Tn mutant | pagL transposon mutant (UWGC:PW8859, PA4661::ISphoA/hah) | 27 |
| phoP::Tn mutant | phoP transposon mutant (UWGC:PW3128, PA1179::ISlacZ/hah) | 27 |
| phoQ::Tn mutant | phoQ transposon mutant (UWGC:PW3131, PA1180::ISlacZ/hah) | 27 |
| pmrB::Tn mutant | pmrB transposon mutant (UWGC:PW9023, PA4777::ISlacZ/hah) | 27 |
| pagP::TnΔpsl mutant | pagP transposon mutant with pslBCD deletion | This study |
| ΔpvdA mutant | pvdA deletion mutant | This study |
| ΔpqsA mutant | pqsA deletion mutant | This study |
| ΔpvdA ΔPpsl mutant | psl promoter and pvdA double-deletion strain | This study |
| ΔpqsA ΔPpsl mutant | psl promoter and pqsA double-deletion strain | This study |
| rhlA::Tn mutant | rhlA transposon mutant (UWGC:PW6886, PA3479::ISphoA/hah) | 27 |
| lasA::Tn mutant | lasA transposon mutant (UWGC:PW4282, PA1871::ISlacZ/hah) | 27 |
| ΔpelA mutant | pelA deletion mutant | 56 |
| 0CM2DB | CF clinical isolate | This study |
| 0CMJ92 | CF clinical isolate | This study |
| 0CPQ3U | CF clinical isolate | This study |
| 0CO5ON | CF clinical isolate | This study |
| 0CMJ8Y | CF clinical isolate | This study |
| 0CM9TX | CF clinical isolate | This study |
| 0CHBKC | CF clinical isolate | This study |
| 0CHBKD | CF clinical isolate | This study |
| 0CH5M4 | CF clinical isolate | This study |
| 0CLLNJ | CF clinical isolate, coisolated with S. aureus | This study |
| 0CLEJ5 | CF clinical isolate, coisolated with S. aureus | This study |
| 0CH7HJ | CF clinical isolate, coisolated with S. aureus | This study |
| 0CHGWG | CF clinical isolate, coisolated with S. aureus | This study |
| 0CGU6K | CF clinical isolate, coisolated with S. aureus | This study |
| 0CI6XZ | CF clinical isolate, coisolated with S. aureus | This study |
| 0CHOI3 | CF clinical isolate, coisolated with S. aureus | This study |
| S. aureus | ||
| USA300 | WT | 57 |
| MSSA | ATCC 29213, methicillin sensitive | ATCC |
| LAC | MRSA | 58 |
| spa::Tn mutant | spa transposon mutant (NE286, NARSA) | 57 |
| Plasmids | ||
| pEX18Gm | For allelic exchange in P. aeruginosa | |
| pΔpqsA | For pqsA deletion | This study |
| pΔpvdA | For pvdA deletion | This study |
| pMpsl-KO1 | For pslBCD deletion | 59 |