TABLE 2.
Expression change of genes involved in pellicle formation or responsive to increased c-di-GMP concentrations in B. cenocepacia J2315 and its mutants as determined by qPCRa
| Pellicle growth |
Planktonic growth |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| cWsp-1 | cWsp-1 | cWsp-2 | WspH-OV | wspR72-VC | cWsp-1 | cWsp-1 | cWsp-2 | cWsp-2 | WspH-OV | WspH-OV | ||
| Gene | Rhamnose | + | − | + | − | − | + | − | + | − | + | − |
| wspH | BCAM0820 | 3.2 | −2.6 | 1.7 | 5.3 | (−0.1) | 3.1 | −2.5 | 1.1 | (0.8) | 11.7 | 4.8 |
| wspA | BCAM0821 | 3.5 | (−2.0) | 6.3 | (1.1) | (0.1) | 2.8 | −1.8 | 4.7 | −1.3 | (0.9) | 1.3 |
| wspE | BCAM0825 | 4.0 | −1.7 | 4.6 | (0.4) | (−0.1) | 2.7 | −2.2 | 6.7 | 2.1 | 1.1 | (0.9) |
| wspF2 | BCAM1162 | (−0.6) | (−0.3) | (−0.3) | (−0.3) | (−1.0) | (−0.6) | (0.1) | (0.5) | (0.3) | (−0.7) | (0.6) |
| wspR | BCAM1161 | (−0.7) | (0.3) | (−0.9) | (-1.0) | (0.8) | (0.3) | (0.2) | (0.5) | (0.4) | (0.0) | (0.9) |
| berA | BCAM1349 | 2.3 | (−0.2) | 2.1 | 4.1 | (0.0) | (0.3) | (−0.4) | ND | ND | (0.2) | (0.9) |
| bepB | BCAM1331 | 1.5 | (−0.2) | (0.9) | 3.1 | (0.1) | −0.5 | (−0.3) | ND | ND | (0.2) | (0.3) |
| manC | BCAM1340 | 2.0 | (−0.3) | 2.4 | 4.3 | (0.1) | (0.0) | (0.2) | ND | ND | (0.3) | 0.5 |
| bcsB | BCAL1389 | −2.1 | (−0.2) | 2.0 | (0.2) | (−0.3) | (0.1) | (0.0) | ND | ND | −2.8 | −1.3 |
| fliC | BCAL0114 | −8.4 | (0.4) | (−2.4) | −9.2 | (−0.2) | (0.0) | (0.4) | ND | ND | −1.7 | −0.9 |
| motA | BCAL0126 | −4.8 | (0.1) | (1.8) | (−1.9) | (0.4) | (−0.3) | (0.0) | ND | ND | −3.1 | −1.3 |
| fimA | BCAL1677 | 3.4 | (−1.1) | 3.4 | 4.5 | (-0.3) | (-0.4) | −1.1 | ND | ND | 4.9 | 4.0 |
| cepI | BCAM1870 | (−0.1) | (−0.1) | (−0.5) | (−1.2) | (−0.1) | (0.5) | −1.0 | ND | ND | (1.4) | (−0.2) |
a
Values are presented as Log2 fold changes compared to VC grown in the same condition. qPCR was performed on three biological replicates, Statistical testing was performed on Log2 fold changes compared to average VC, values p ≤ 0.05 were reported as significant. Values in brackets: not significant. ND: not determined. The position of mutations for cWsp-1, cWsp-2 and wspR72 is depicted in Fig. 1.