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. 2022 May 3;13:877404. doi: 10.3389/fpls.2022.877404

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Copyright © 2022 He, Zhang, Xu, Chen and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of BnRALF10 reduced resistance to S. sclerotiorum in oilseed rape. (A) Photobleaching phenotype of BnPDS silencing in oilseed rape employing pTY-based VIGS. Empty vector pTY-S was used as a control. (B) Efficient silencing of BnRALF10 in leaves as manifested by the dramatically reduced level of BnRALF10 transcript, which was detected by qRT-PCR analysis using B. napus actin (BnActin7) as reference gene. (C) Representative disease symptoms (left), lesion areas (middle) and fungal biomass (right) of BnRALF10-silenced leaves at 24 h post inoculation with S. sclerotiorum. Scale bar: 1 cm. Data are statistically analyzed by Student’s t-test (n = 10) and shown as the mean ± SE. The asterisks indicate significant differences (*P ≤ 0.05, ***P ≤ 0.001, ****P ≤ 0.0001).