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. Author manuscript; available in PMC: 2022 May 17.
Published in final edited form as: Methods Mol Biol. 2021;2265:305–321. doi: 10.1007/978-1-0716-1205-7_23

Fig. 4:

Fig. 4:

Immune capture of CD3+ exosomes and detection of the exosome cargo by on-bead flow cytometry. After exosome isolation from plasma by mini-SEC, immune capture with biotinylated anti-CD3 mAb antibody on beads separated CD3+ from uncaptured CD3neg exosomes. Proteins carried by CD3+ exosomes on beads are detected by using labeled mAbs specific for these proteins and on-bead flow cytometry. Non-captured CD3neg exosomes can be captured on beads using biotinylated ant-CD63 antibody for detection. The figure is adapted from Theodoraki et al. [15] and reproduced with permission of the Wiley Publishing Company.