Verification of target gene CXCL12 in GC cells. (a) The expression of CXCL12 at the mRNA level in BGC-823, MGC-803, and SGC-7901 cells following miR-1273h-5p mimic transfection was evaluated using RT-PCR. miR-1273h-5p mimic group revealed a significant decrease compared to the negative group in three GC cells. (b, d, and f) The expression of CXCL12 at the protein level in BGC-823, MGC-803, and SGC-7901 cells following miR-1273h-5p mimic transfection determined using Western blot. The expression of CXCL12 in mimics revealed a significant reduction of BGC-823 and SGC-7901cells by contrast with negative control. (c, e, and h) Protein electrophoretic bands of BGC-823, MGC-803, and SGC-7901 cells, respectively. All data were represented as M ± SD from three independent experiments compared with the negative control group, *P < 0.05, **P < 0.01.