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. 2022 May 16;17(1):930–946. doi: 10.1515/med-2022-0486

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© 2022 Yi-Chen Wang et al., published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 International License.

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Figure A1 — Firefly luciferase plasmids of target gene (CXCL12). (a) During the co-transfection process, experimental grouping has been completed, including miR-1273h-5p(negative con)-CXCL12(negative con), miR-1273h-5p(negative con)-CXCL12(wide type 1 or 2), miR-1273h-5p(negative con)-CXCL12(mutant 1 or 2), miR-1273h-5p(mimic)-CXCL12(negative con), miR-1273h-5p(mimic)-CXCL12(wide type 1 or 2), and miR-1273h-5p(mimic)-CXCL12(mutant 1 or 2). (b) Firefly luciferase negative control plasmids, which did not carry the gene sequence. (c) Base sequences of CXCL12 wild type 1 and mutation 1 and assembly sites with plasmid vectors. (d) Base sequences of CXCL12 wild type 2 and mutation 2 and assembly sites with plasmid vectors. The sequence of junction bases with miRNA is bold.