Figure 2.

Generation of the transgenic CYP21A2 mouse model. Schematic presentation of homologous recombination of the targeting vector carrying the human CYP21A2 sequence (dark blue) flanked by 1 kb mouse homology arms (light blue), with the endogenous Cyp21a1 gene locus (light blue): wild-type Cyp21a1 gene locus (top), targeting HDR donor plasmid containing fully humanized CYP21A2 sequence and a PGK-Neo selection cassette flanked by LoxP sites (middle with twice zoom-in), and transgenic gene locus (bottom) after homologous recombination and LoxP sites excision by Cre recombinase, leaving a single LoxP as a genomic scar.