Figure 4.

Adrenal gene expression in h-Hom CYP21A2 mice compared to WT mice. Expression of Cyp21a1 (A), CYP21A2 (B), Cyp11a1 (C), Star (D), Cyp11b1 (E), Cyp11b2 (F), Hsd3b2 (G) and Nr5a1 (H) in adrenal glands from male and female 10-wk-old WT and h-Hom mice was determined by qRT-PCR. Relative mRNA expression levels were normalized to β-actin expression (Actb). Experiments were performed in triplicates, and data are expressed as means with SEM. Statistical significance was determined by Mann-Whitney U test, *P < 0.05, **P < 0.01 and ***P < 0.001; n = 7.