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Immunofluorescence staining of cryosections of h-Hom CYP21A2 mice compared to WT mice. For specific staining adrenal cryosections from h-Hom and WT mice were incubated with anti-tyrosine hydroxylase antibody (A), anti-Sf-1 antibody (B + C) or anti-Star antibody (D) and visualized by a secondary Cy3-fluorescent labeled antibody (red). Nuclei were stained with 4′-6-diamidino-2-phenylindole (DAPI; blue). Scale bar: 200 µM (A, B) and 50 μm (C, D).
